Peptide Reconstitution Calculator

The two main types of compounds you may reconstitute are peptides and hormones. They differ in how they are measured and dosed.

Peptides are short chains of amino acids measured by weight in milligrams (mg) or micrograms (mcg). Because they are synthetically produced, their mass is consistent between batches. Examples include BPC-157, TB-500, and Semaglutide.

Hormones such as hCG and hGH are measured in International Units (IU) — a standardized measure of biological activity, not mass. IU ensures consistent dosing based on the compound's actual effect rather than its weight.

Peptide vials are labeled in milligrams (mg), but individual doses are often measured in micrograms (mcg). There are 1,000 mcg in 1 mg.

For example, a 10 mg vial of BPC-157 dosed at 250 mcg per injection would provide 40 doses. The calculator handles the mg/mcg conversion automatically.

The amount printed on a peptide vial is the labeled quantity — not necessarily the actual fill. Research peptide vials commonly deviate from their label.

The Certificate of Analysis (COA) for your manufacturing batch is the most reliable reference for estimating the actual fill amount. Switching suppliers or batches can result in different fill amounts even with identical labels.

Bacteriostatic Water (BAC)

Sterile water with 0.9% benzyl alcohol as a preservative. The most common choice — allows multi-dose use over several weeks because the preservative inhibits bacterial growth.

Sterile Water

Pure water without preservatives. Once used to reconstitute a vial, the solution should ideally be used within 24 hours or refrigerated and used promptly.

Sodium Chloride (0.9%)

Saline solution sometimes required for specific compounds. Check your product documentation for solvent requirements.

Concentration is the amount of compound per unit of liquid, measured in mg/mL.

More solvent = lower concentration = larger draw volume per dose.

Less solvent = higher concentration = smaller draw volume per dose.

The amount of solvent you add is a balance: too little makes precise dosing difficult with small syringes, while too much may require drawing more liquid than your syringe can hold.

The administration route determines how the reconstituted solution is delivered to your body. Each method has different requirements for measuring and delivering each dose.

Injection uses a syringe to deliver a precise volume of liquid subcutaneously or intramuscularly.

Intranasal uses a nasal spray bottle to deliver doses as sprays into the nasal passages. Dosing is based on the number of sprays rather than a liquid volume measurement.

Your desired dose and the solution's concentration together determine the draw volume — the amount of liquid to draw into the syringe for each injection.

Different syringe sizes (0.3 mL, 0.5 mL, 1 mL, 3 mL) have different capacities and graduation intervals. Choosing a syringe that closely matches your draw volume allows for more precise measurement.

0.3 mL Syringe

Best for very small doses. Graduation marks often represent 0.005 mL (half a unit), offering the highest precision.

0.5 mL Syringe

A good balance of precision and capacity. Common for most peptide doses. Graduations are typically every 0.01 mL (1 unit).

1 mL Syringe

A standard insulin syringe size. Suitable for larger draw volumes. Graduations are typically every 0.01 mL (1 unit).

3 mL Syringe

For large volumes. Often used for reconstitution when filling vials with multiple mL of solution. Less precise for small doses due to wider graduation spacing.

Graduation spacing can vary by manufacturer — always check the markings on your specific syringe.

Most insulin syringes use the U-100 standard, meaning 100 units = 1 mL. Each unit mark equals 0.01 mL.

Some syringes show only units (0–100), others show mL (0–1.0 mL), and some show both. Use the scale toggle in the calculator to match your syringe's markings.

For example, a draw of 0.25 mL is the same as 25 units on a U-100 syringe.